Author

Edward J. King

University of California, Irvine

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Molecular Biology· 2 Synthetic Biology· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 3 Approach:experimental assay· 3 Research Focus:protein design· 3 Research Focus:drug response / drug discovery· 1 Assay:proteomics· 1 Approach:statistical model· 1

Frequent Co-Authors

Han LiiD· 4 shared Justin B. SiegeliD· 3 shared William B. BlackiD· 3 shared Youtian CuiiD· 3 shared Derek AspacioiD· 2 shared Emma LuuiD· 2 shared Jin Young KimiD· 2 shared Linyue Zhang· 2 shared

Activity by Year

2020-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

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4 shared papersMolecular Biology

Han LiORCID

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Author profile →

4 papers

Recent·Most cited

Reprogramming the Rossmann Fold Signature Motif Creates Orthogonal Redox Biocatalysts

Yu Ping, Jin Young Kim, Minh-Anh L. Dinh, Emma Luu, Suphanida Worakaensai, Shiding Zhang, William B. Black, Emma Monge, Hans Jefferson C. Ng, Sean Perea, Yulai Zhang, Youtian Cui, Edward J. King, Justin B. Siegel, Han Li

The paper reprograms the Rossmann fold motif to create NMN+-specific enzymes that avoid NAD(P)/H interactions, achieving high specificity and productivity in biotransformation.

Nature Communications · Sep 12, 2026Peer-reviewedMolecular Biology

Engineering the key central metabolic enzyme to utilize a non-canonical redox cofactor

Jin Young Kim, Edward J. King, Youtian Cui, Linyue Zhang, Justin B. Siegel, Han Li

This paper engineers Escherichia coli GapA to use NMN+ instead of NAD+, achieving a high cofactor specificity switch through rational design and mutagenesis.

bioRxiv · May 28, 2025 · PreprintMolecular Biology

Shifting Redox Reaction Equilibria on Demand Using an Orthogonal Redox Cofactor

Derek Aspacio, Yulai Zhang, Youtian Cui, Emma Luu, Edward J. King, William B. Black, Sean Perea, Qiang Zhu, Yongxian Wu, Ray Luo, Justin B. Siegel, Han Li

This paper introduces Nox Ortho, an enzyme that enables precise control of redox reactions using NMN+ as a cofactor, achieving high specificity and enabling chiral-pure product synthesis.

Nature Chemical Biology · Aug 13, 2024Peer-reviewedSynthetic Biology

Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor

William B. Black, Derek Aspacio, Danielle Bever, Edward J. King, Linyue Zhang, Han Li

This paper engineers Escherichia coli to biosynthesize nicotinamide mononucleotide (NMN+) for use in cell-free biosynthesis, achieving a 130-fold increase in intracellular NMN+ levels.

Microbial Cell Factories · Jul 27, 2020Peer-reviewedSynthetic Biology