Matthew J. Gust

Integrative Single-cell and Spatial Transcriptomic Analysis of Osteosarcoma Reveals Conserved and Distinct Ecosystems Across Sites and Species

This study integrates single-cell and spatial transcriptomic data across species to identify conserved and distinct tumor ecosystems in osteosarcoma, revealing canine models as more faithful and highlighting ECM signaling in metastatic lesions.

SCANBIT facilitates identification of tumor cell populations in scRNAseq data using pseudobulked SNV calls

SCANBIT identifies tumor cell populations in scRNA-seq data by using pseudobulked SNV calls, showing that mutational burden affects identification accuracy.

Metastasis-initiating osteosarcoma subpopulations establish paracrine interactions with both lung and tumor cells to create a metastatic niche

This study identifies metastasis-initiating osteosarcoma subpopulations that interact with lung and tumor cells to form a metastatic niche, highlighting tumor-stromal interactions as key drivers of metastasis.