Epigenetics & ChromatinMolecular BiologyPeer-reviewed journal article
Transcription-dependent DNA methylation at the imprinted Zrsr1-DMR
Keiichiro JohiD, Fumikazu Matsuhisa, Shuji KitajimaiD, Kenichi NishiokaiD, Ken Higashimoto, Hitomi Yatsuki, Tomohiro KonoiD, Haruhiko KosekiiD, Hidenobu SoejimaiD
Abstract
Zrsr1 is a paternally expressed imprinted gene located in the first intron of Commd1, and the Zrsr1 promoter resides in a differentially methylated region (DMR) that is maternally methylated in the oocyte. However, a mechanism for the establishment of the methylation has remained obscure. Commd1 is transcribed in the opposite direction to Zrsr1 with predominant maternal expression, especially in the adult brain. In this study, we found Commed1 transcribed through the DMR in the growing oocyte. Zrsr1-DMR methylation was abolished by the prevention of Commd1 transcription. This result indicated that methylation at the Zrsr1-DMR was transcription-dependent.
- organism: mouse
- assay: DNA methylation
- research focus: gene regulatory network inference
- approach: experimental assay
- Citations
- 22
- FWCI
- 1.07
- Field percentile
- top 25%
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