Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R

Tal M. Dankovich; Rahul Kaushik; Linda H. M. Olsthoorn; Gabriel Cassinelli Petersen; Philipp Emanuel Giro; Verena Kluever; Paola Agüi‐Gonzalez; Katharina Grewe; Guobin Bao; Sabine Beuermann; Hannah Abdul Hadi; José Doeren; Simon Klöppner; Benjamin H. Cooper; Alexander E. Dityatev; Silvio O. Rizzoli

doi:10.1038/s41467-021-27462-7

NeuroscienceNature CommunicationsDec 8, 2021

Peer-reviewed journal article

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R

Tal M. Dankovich iD, Rahul Kaushik iD, Linda H. M. Olsthoorn iD, Gabriel Cassinelli Petersen iD, Philipp Emanuel Giro, Verena Kluever iD, Paola Agüi‐Gonzalez iD, Katharina Grewe, Guobin Bao iD, Sabine Beuermann, Hannah Abdul Hadi, José Doeren iD, Simon Klöppner, Benjamin H. Cooper iD, Alexander E. Dityatev iD, Silvio O. Rizzoli iD

Abstract

The brain extracellular matrix (ECM) assembles around neurons and synapses, and is thought to change only rarely, through proteolysis and renewed protein synthesis. We report here an alternative ECM remodeling mechanism, based on the recycling of ECM molecules. We found that a key ECM protein, Tenascin-R, is frequently endocytosed, and later resurfaces, preferentially near synapses. The TNR molecules complete this cycle within [~]3 days, in an activity-dependent fashion.

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