High-throughput deep tissue two-photon microscopy at kilohertz frame rates
Abstract
Strategies to image biological phenomena at millisecond time scales are generally technically challenging and suffer from compromises between imaging field-of-view, depth penetration and excitation efficiency in thick tissue. We present a simple and cost-effective solution that enables a conventional video-rate two-photon microscope (2PM) to perform 2D scanning at kilohertz frame rates, while preserving all the benefits of standard 2PM, which we demonstrate by imaging neurovascular dynamics in mouse brains.
02 /Specification & Classification
Click any tag to browse matching papers- Organism
- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Kilohertz frame-rate two-photon tomography
Abbas Kazemipour, Ondřej Novák, … Kaspar Podgorski·Nature MethodsPeer-reviewed·Jul 29, 2019
- Kilohertz-rate two-photon voltage imaging of population dynamics in vivo
Min Zhang, Shoupei Liu, … Bo Li·bioRxiv·Mar 27, 2026
- High-Speed Neural Imaging with Multiplexed Miniature Two-Photon Microscopy (M-MINI2P)
Zixiao Zhang, Shing-Jiuan Liu, … Weijian Yang·bioRxiv·Mar 4, 2025
- Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds
Mitchell Clough, Anderson Chen, … Jerry L. Chen·Nature CommunicationsPeer-reviewed·Nov 17, 2021
- Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Jianglai Wu, Yajie Liang, … Na Ji·Nature MethodsPeer-reviewed·Mar 2, 2020
- A Large Field-of-view, Single-cell-resolution Two- and Three-Photon Microscope for Deep Imaging
Aaron T. Y. Mok, Tianyu Wang, … Chris Xu·bioRxiv·Nov 14, 2023