Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization

Phenotypic and genetic diversity is found in varying prevalence in clinical populations where beneficial adaptations enable the bacteria to avoid recognition and eradication by the host immune system. This study aimed to investigate the presence of Pseudomonas aeruginosa in chronic venous leg ulcers wounds over an 8-week time course. This was performed using genomic and phenotypic approaches to understand the survival and persistence of Pseudomonas strains. The findings of this study show that the two patients were colonized with a recurring P. aeruginosa genotype with only minor phenotypic differences and few SNP differences, suggesting that the Pseudomonas isolates present in the wound can survive and proliferate in the hosts hostile environment. The results provided from this study will allow us to understand clinically important traits in P. aeruginosa and provide insight into the dynamics and adaptations (or the lack thereof) in chronic wounds. HighlightsO_LIEach patient is colonised overtime with a unique MLST C_LIO_LIThe total number of detected SNPs was 18 and 20 within each patient, respectively C_LIO_LIOne MLST belonged to the sequence type ST132, often observed in clinical isolates, whereas the other belonged to ST3244, previously seen inenvironmental isolates C_LI