Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes
B. Sana, D. Ke, E. H. Y. Li, T. Ho, J. Seayad, H. A. Duong, F. Ghadessy
Abstract
We demonstrate novel in vitro halogenation of peptides by halogenase enzymes, and identify the (G/S)GW motif (HaloTryp Tag) as a preferred substrate. We further derive PyrH halogenase mutants showing improved halogenation of the HaloTryp Tag, both as a free peptide and when genetically fused to model proteins.
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