A chemogenetic approach for optical monitoring of voltage in neurons

Mayya Sundukova; Efthymia Prifti; Annalisa Bucci; Kseniia Kirillova; Joana Serrao; Luc Reymond; Miwa Umebayashi; Ruud Hovius; Howard Riezman; Kai Johnsson; Paul A. Heppenstall

doi:10.1002/anie.201812967

NeuroscienceAngewandte Chemie International EditionDec 20, 2018

Peer-reviewed journal article

A chemogenetic approach for optical monitoring of voltage in neurons

Mayya Sundukova iD, Efthymia Prifti, Annalisa Bucci iD, Kseniia Kirillova, Joana Serrao, Luc Reymond iD, Miwa Umebayashi iD, Ruud Hovius iD, Howard Riezman iD, Kai Johnsson iD, Paul A. Heppenstall iD

Abstract

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for interrogation of the cellular and molecular logic of the nervous system. Here we describe a Semisynthetic Tethered Voltage Indicator (STeVI1) based upon Nile Red that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and subthreshold activity in neurons.

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