Non-invasive approach for endoluminal biopsy coupled with single-cell proteomics allows for immune characterization of intracranial aneurysms

Joseph Paul Antonios; Batur Gültekin; Brianna Carusillo Theriault; Kanat Yalcin; Danielle F. Miyagishima; Nana Adenu‐Mensah; Nanthiya Sujijantarat; Andrew B. Koo; Joseph O Haynes; Paul Anthony Cedeño; Michele H. Johnson; Ryan Matthew Hebert; Charles Christian Matouk; Tanyeri Barak; Murat Günel

doi:10.1101/2025.02.17.638658

PhysiologybioRxivFeb 17, 2025

Preprint

Non-invasive approach for endoluminal biopsy coupled with single-cell proteomics allows for immune characterization of intracranial aneurysms

Joseph Paul Antonios iD, Batur Gültekin iD, Brianna Carusillo Theriault iD, Kanat Yalcin iD, Danielle F. Miyagishima iD, Nana Adenu‐Mensah, Nanthiya Sujijantarat iD, Andrew B. Koo iD, Joseph O Haynes iD, Paul Anthony Cedeño, Michele H. Johnson iD, Ryan Matthew Hebert iD, Charles Christian Matouk iD, Tanyeri Barak iD, Murat Günel iD

Abstract

The immune regulatory mechanisms driving the stability, growth, and rupture of intracranial aneurysms (IAs) remain incompletely understood. In this study, we employ endoluminal biopsy with single-cell proteomics to comprehensively profile the immune landscape of IAs across their pathologic states. Our findings reveal distinct immune signatures associated with aneurysm states. Stable, i.e. non-growing unruptured, IAs exhibit a balanced immune cell composition. Ruptured IAs are marked by significant neutrophil predominance. Notably, we highlight key immune markers in aneurysm instability, offering new insights into immune drivers of aneurysm progression. These findings provide a foundation for immune-targeted, non-invasive therapeutic strategies aimed at targeting IAs and preventing rupture.

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