Droplet-compatible single-cell DNA methylation sequencing with PreTIC
Sheng ZhangiD, Faming WangiD, Y Zhang, Keng-Jung Lee, Carl EngmaniD, Hong-Zhang He, Yong Fan, Si-Yang Zheng
Abstract
Single-cell DNA methylation sequencing has remained technically specialized due to challenges of interfacing conversion chemistry with droplet microfluidics. We introduce pre-tagmentation in situ conversion (PreTIC), which enables whole-methylome profiling on a commercial droplet platform using off-the-shelf reagents. With PreTIC, we produce over 13,000 single-cell methylomes from fixed frozen cells in two days and resolve cell-specific epigenetic variation in human peripheral blood mononuclear cells.
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