An optimized toolkit for precision base editing
Maria Paz Zafra, Emma M Schatoff, Alyna KattiiD, Miguel ForondaiD, Marco Breinig, Anabel Y. Schweitzer, Amber Simon, Teng Han, Sukanya GoswamiiD, Emma Montgomery, Jordana Thibado, Francisco J. Sánchez-Rivera, Junwei Shi, Christopher R Vakoc, Scott W Lowe, Darjus F. Tschaharganeh, Lukas E Dow
Abstract
CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single base pair resolution. By re-engineering both DNA and protein sequences, we developed a collection of constitutive and inducible base editing vector systems that dramatically improve the ease and efficiency by which single nucleotide variants can be created. This new toolkit is effective in a wide range of model systems, and provides a means for efficient in vivo somatic base editing.
02 /Specification & Classification
Click any tag to browse matching papers- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- EditR: A novel base editing quantification software using Sanger sequencing
Mitchell G. Kluesner, Derek A. Nedveck, … Branden S. Moriarity·The CRISPR JournalPeer-reviewed·Jun 1, 2018
- Efficient DNA base editing via an optimized DYW-like deaminase
Jiyeon Kweon, Soomin Park, … Yongsub Kim·bioRxiv·May 15, 2024
- Base editing generates substantial off-target single nucleotide variants
- Web-based design and analysis tools for CRISPR base editing
Gue‐Ho Hwang, Jeongbin Park, … Sangsu Bae·BMC BioinformaticsPeer-reviewed·Dec 1, 2018
- The pAblo·pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Pseudomonas species
Ekaterina Kozaeva, Zacharias S. Nielsen, … Pablo I. Nikel·Nucleic Acids ResearchPeer-reviewed·Jan 2, 2024
- High-precision CRISPR-Cas9 base editors with minimized bystander and off-target mutations
Jason M. Gehrke, Oliver Cervantes, … J. Keith Joung·bioRxiv·Mar 1, 2018