Author

Walker S. Lahr

University of MinnesotaORCID 0000-0002-3288-3843Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 2 Genetics· 2 Genomics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 4 Assay:CRISPR screen· 3 Research Focus:gene regulatory network inference· 3 Research Focus:cell type annotation· 2 Approach:genetic perturbation / CRISPR· 2 Research Focus:drug response / drug discovery· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Beau R. WebberiD· 5 shared Branden S. MoriarityiD· 5 shared Mitchell G. KluesneriD· 4 shared Nicholas J. SlipekiD· 3 shared Alexandria K. Gilkey· 2 shared Cara-lin LonetreeiD· 2 shared Emily J. PomeroyiD· 2 shared Erin M. Stelljes· 2 shared

Activity by Year

2018-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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5 shared papersBioengineering

Beau R. WebberORCID

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Author profile →

5 papers

Recent·Most cited

A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells

Joseph G. Skeate, Nicholas J. Slipek, Walker S. Lahr, Shambojit Roy, Bryce J. Wick, Bibekananda Kar, Jae-Woong Chang, Erin M. Stelljes, Alexandria K. Gilkey, Prateek P. Thenge, Miechaleen D. Diers, Joshua B. Krueger, Ethan M. Niemeyer, Cara-lin Lonetree, Mitchell G. Kluesner, , Beau R. Webber

This paper develops a base editing platform to efficiently and safely engineer CAR T cells with multiple gene knockouts and insertions.

Molecular Therapy · Jul 18, 2026Peer-reviewedBioengineering

Precision Enhancement of CAR-NK Cells through Non-Viral Engineering and Highly Multiplexed Base Editing

Minjing Wang, Joshua B. Krueger, Alexandria K. Gilkey, Erin M. Stelljes, Mitchell G. Kluesner, Emily J. Pomeroy, Joseph G. Skeate, Nicholas J. Slipek, Walker S. Lahr, Patricia N. Claudio Vázquez, Yueting Zhao, Jason B Bell, Kendell Clement, Ella J. Eaton, Kanut Laoharawee, , Branden S. Moriarity

This study enhances CAR-NK cell functionality using non-viral base editing and transposon integration, showing improved therapeutic potential in Burkitt's lymphoma models.

Journal for ImmunoTherapy of Cancer · May 1, 2025Peer-reviewedBioengineering

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Beau R. Webber, Cara-lin Lonetree, Mitchell G. Kluesner, Matthew J. Johnson, Emily J. Pomeroy, Miechaleen D. Diers, Walker S. Lahr, Garrett M. Draper, Nicholas J. Slipek, Branden A. Smeester, Klaus N. Lovendahl, Amber N. McElroy, Wendy Ryan Gordon, Mark J. Osborn, Branden S. Moriarity

This study develops a method to efficiently edit multiple genes in human T cells using base editors, reducing off-target effects and improving safety for immunotherapy.

Nature Communications · Nov 19, 2019Peer-reviewedGenetics

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

Matthew J. Johnson, Kanut Laoharawee, Walker S. Lahr, Beau R. Webber, Branden S. Moriarity

This paper describes optimized CRISPR/Cas9 genome engineering of primary human B cells, achieving high-efficiency gene knockout and site-specific integration.

Scientific Reports · Aug 8, 2018Peer-reviewedGenomics

EditR: A novel base editing quantification software using Sanger sequencing

Mitchell G. Kluesner, Derek A. Nedveck, Walker S. Lahr, John Garbe, Juan E. Abrahante, Beau R. Webber, Branden S. Moriarity

EditR quantifies base editing efficiency from Sanger sequencing data, offering accuracy and cost-effectiveness compared to existing methods.

The CRISPR Journal · Jun 1, 2018Peer-reviewedGenetics