Cell Reports MethodsBiophysicsPeer-reviewed journal article
Feature-Driven Whole-Tissue Imaging with Subcellular Resolution
Jinlong Lin, Zach MariniD, Xiaoding WangiD, Hazel M. BorgesiD, Qionghua SheniD, Pierre-Emmanuel N'GuettaiD, Xuemei Luo, Baylee A. PorteriD, Yuanyuan XueiD, Md Torikul IslamiD, Tai Ngo, Doreen Idonije, Seweryn GałeckiiD, Arin B. AuroraiD, Hu ZhaoiD, Suzanne Daniela ConzeniD, Sean J. MorrisoniD, Shuang LiangiD, Zhenyu ZhongiD, Lori L. O’BrieniD, Kevin M. DeaniD
Abstract
Understanding how subcellular structures and events shape tissue-wide phenomena remains a central challenge in biology. We present Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to submicron (~300 nm) isotropic resolution for targeted imaging. This versatility enables comprehensive studies of hierarchical organization and spatially complex processes, including mapping neuronal circuits in rat brains, visualizing glomerular innervation in mouse kidneys, and examining metastatic tumor microenvironments. By bridging subcellular to tissue-level scales, MCT-ASLM offers a powerful method for unraveling how local events contribute to global biological phenomena.
- organism: rat
- organism: mouse
- assay: imaging
- research focus: cell type annotation
- research focus: spatial domain detection
- approach: microscopy & imaging analysis
- Citations
- 4
- FWCI
- 1.51
- Field percentile
- top 17%
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