R. Fiolka

The Vps13-like protein BLTP2 is pro-survival and regulates phosphatidylethanolamine levels in the plasma membrane to maintain its fluidity and function

This study identifies BLTP2 as a pro-survival protein that regulates phosphatidylethanolamine levels in the plasma membrane to maintain its fluidity and function, linking it to cancer aggressiveness.

In vivo profiling of site-specific human cancer cell states in zebrafish

The paper uses zebrafish xenografts and computer vision to study how Ewing Sarcoma cell morphology changes in different sites and under EWSR1-FLI1 expression variations.

A systematic and quantitative comparison of lattice and Gaussian light-sheets

This study compares lattice and Gaussian light-sheets quantitatively, finding they are similar in thickness, confocal parameter, and propagation length.

Extended depth of focus multiphoton microscopy via incoherent pulse splitting

The paper introduces a phase mask to extend depth of focus in multiphoton microscopy with minimal lateral resolution loss and demonstrates its effectiveness in imaging fixed brain samples.

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

The paper investigates the role of actin-binding proteins in cell spreading on 2D and 3D substrates, finding that Arp2/3-vinculin binding is critical for 3D spreading but not 2D.

A unified role for membrane-cortex detachment during cell protrusion initiation

The paper shows that membrane-cortex detachment is essential for initiating both pressure-based and actin-based protrusions, challenging the Brownian ratchet model and unifying protrusion regulation.

Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

This paper introduces ctASLM, a light-sheet microscopy technique with sub-micron isotropic resolution and large-scale imaging, enabling automated cell and subcellular feature detection in cleared tissues.

An image-based assay to quantify changes in proliferation and viability upon drug treatment in 3D microenvironments

This paper introduces an image-based assay to measure drug effects on cell proliferation and viability in 3D microenvironments, showing 3D single cells are as effective as spheroids for drug screening.

Cell morphological motif detector for high-resolution 3D microscopy images

The paper introduces a morphological motif detector for 3D microscopy images to analyze cell shape dynamics and signaling.