bioRxivMolecular BiologyPreprint
Enhanced tissue penetration of antibodies through pressurized immunohistochemistry
Roberto Fiorelli, Gurpaul Singh Sidhu, Arantxa Cebrián‐SillaiD, Ernesto Luna MelendeziD, Shwetal V. MehtaiD, José Manuel García‐VerdugoiD, Nader SanaiiD
Abstract
To address the inefficiency of passive diffusion for antibody penetration in thick tissue samples, which limits clearing-technique applications, we developed a versatile and simple device to perform antibody incubation under increased barometric pressure. Pressurized immunohistochemistry greatly improves the uniformity, intensity, and depth of fluorescent immunostaining in thick human and mouse brain samples. Furthermore, pressurized immunohistochemistry substantially decreases the time required for classic staining of thin sections. SUBMISSION CATEGORYNew Results
- Citations
- 9
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue
T. J. Zwang, R. E. Bennett, … B. T. Hyman·bioRxiv·May 25, 2022
- A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
Kenta Yamauchi, Masato Koike, Hiroyuki Hioki·Communications BiologyPeer-reviewed·Jun 18, 2025
- Accelerated clearing and molecular labeling of large tissue samples using magnetohydrodynamic force
J. R. Dwyer, M. Desmond Ramirez, … Joseph F. Bergan·bioRxiv·Oct 29, 2019
- High-plex protein imaging across thick tissue by synchronized amplification
Kuanwei Sheng, Hanquan Su, … Peng Yin·bioRxiv·Oct 14, 2026
- CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue
Sarah Woelfle, Dhruva Deshpande, … Michael Schön·BMC BiologyPeer-reviewed·May 23, 2023
- High-Resolution 3D Fluorescent Imaging of Intact Tissues
D. El-Nachef, A. M. Martinson, … W. R. MacLellan·bioRxiv·Nov 26, 2019