Human organoid systems reveal in vitro correlates of fitness for SARS-CoV-2 B.1.1.7
M. M. Lamers, T. I. Breugem, A. Z. Mykytyn, Y. Wang, N. Groen, K. Knoops, D. Schipper, J. van der Vaart, C. D. Koopman, J. Zhang, D. C. Wu, P. B. van den Doel, T. Bestebroer, C. C. GeurtsvanKessel, P. J. Peters, M. J. Muraro, H. Clevers, N. C. Wu, B. L. Haagmans
Abstract
A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2. One-Sentence SummaryBritish SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro.
02 /Specification & Classification
Click any tag to browse matching papers- Organism
- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Enhanced fitness of SARS-CoV-2 variant of concern B.1.1.7, but not B.1.351, in animal models
L. Ulrich, N. J. Halwe, … C. Benarafa·bioRxiv·Jun 28, 2021
- Low dose inocula of SARS-CoV-2 B.1.1.7 variant initiate more robust infections in the upper respiratory tract of hamsters than earlier D614G variants
B. W.-Y. Mok, H. Liu, … H. Chen·bioRxiv·Apr 19, 2021
- Replicative fitness SARS-CoV-2 20I/501Y.V1 variant in a human reconstituted bronchial epithelium
F. Touret, L. Luciani, … X. de Lamballerie·bioRxiv·Mar 22, 2021
- Relative infectivity of the SARS-CoV-2 Omicron variant in human alveolar cells
- SARS-CoV-2 Omicron efficiently infects human airway, but not alveolar epithelium
M. M. Lamers, A. Z. Mykytyn, … B. L. Haagmans·bioRxiv·Jan 19, 2022
- UK B.1.1.7 variant exhibits increased respiratory replication and shedding in nonhuman primates
K. Rosenke, F. Feldmann, … H. Feldmann·bioRxiv·Jun 11, 2021