bioRxivMicrobiology
Topoisomerase III-beta is required for efficient replication of positive-sense RNA viruses
K. R. Prasanth, M. Hirano, W. S. Fagg, E. McAnarney, S. Chan, X. Xie, A. Hage, C. Pietzsch, A. Bukreyev, R. Rajsbaum, P.-Y. Shi, M. Bedford, S. Bradrick, V. D. Menachery, M. A. Garcia-Blanco
Abstract
Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-{beta} (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.
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