bioRxivMolecular BiologyPreprint
Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by a widely-used RT-PCR assay
Manu VanaerschotiD, Sabrina A ManniD, James T. WebberiD, Jack KammiD, Sidney M. BelliD, John BelliD, Si Noon Hong, Minh Phuong Nguyen, Lienna Y. Chan, Karan D. BhattiD, Michelle TaniD, Angela M. DetweileriD, Alex Espinosa, Wesley C. H. WuiD, Joshua BatsoniD, David DynermaniD, Debra A. WadfordiD, Andreas S. PuschnikiD, Norma F NeffiD, Vida AhyongiD, Steve A. MilleriD, Patrick Ayscue, Cristina M. TatoiD, Simon Paul, Amy L. KistleriD, , Emily D. CrawfordiD
Abstract
We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets, preferably in at least 2 genes, for robust SARS-CoV-2 detection. Article Summary LineA SARS-CoV-2 variant that occurs worldwide and has spread in California significantly affects diagnostic sensitivity of an N gene assay, highlighting the need to employ multiple viral targets for detection.
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