Enzyme Catalysis and Immobilization

Towards XNA molecular biology: Bacterial cell display as a robust and versatile platform for the engineering of low affinity ligands and enzymes

This paper introduces SNAP as a quantitative reporter for bacterial cell display, enabling the development of a versatile platform for engineering low affinity ligands and enzymes.

A de novo peroxidase is also a promiscuous yet stereoselective carbene transferase

The paper demonstrates that a de novo-designed peroxidase, C45, also functions as a promiscuous and stereoselective carbene transferase, enabling efficient ring expansion of aromatic heterocycles.

Computational redesign of a PETase for plastic biodegradation by the GRAPE strategy

This paper computationally redesigns PETase to improve its stability and PET degradation efficiency using the GRAPE strategy.

Engineered Enzymes that Retain and Regenerate their Cofactors Enable Continuous-Flow Biocatalysis

The paper describes engineered enzymes that retain and regenerate cofactors, enabling continuous-flow biocatalysis.

Golden Mutagenesis: An efficient multi-site saturation mutagenesis approach by Golden Gate cloning with automated primer design

The paper introduces Golden Mutagenesis, a method for creating mutagenesis libraries with automated primer design, enabling rapid and cost-effective multi-site saturation mutagenesis.

Thermostability improvement of Aspergillus awamori glucoamylase via directed evolution of its gene located on episomal expression vector in Pichia pastoris

This study improves the thermostability of Aspergillus awamori glucoamylase through directed evolution and identifies key mutations that enhance or reduce thermal stability.

Simulation-Based protein engineering of R. erythropolis NADH-FMN Oxidoreductase

This study improves the specificity of the DszD enzyme through mutagenesis guided by structural predictions and simulations, resulting in increased enzyme activity.