Discover, rate, and verify biology preprints.

A global post-transcriptional regulator directs Acinetobacter baumannii carbon preference and gut colonization

This study identifies CsrA as a global regulator of carbon preference and gut colonization in Acinetobacter baumannii, revealing its role in repressing arginine/ornithine catabolism and enhancing competitive fitness.

Metagenomic Insights into the Fecal Microbiome, Resistome, and Mobilome of Beef Cattle in Cow-Calf Operations in Florida, United States

The paper analyzes the fecal microbiome, resistome, and mobilome of beef cattle in Florida, finding differences in ARG abundance between cows and calves and a notable proportion of ARGs on plasmids.

Gene-resolved metagenomics reveals partitioned nitrogen transformation potential in a landfill leachate sequencing batch reactor microbiome

This study uses gene-resolved metagenomics to analyze nitrogen transformation potential in a landfill leachate SBR microbiome, revealing gene distribution across taxa and potential nitrogen retention pathways.

Functional Conservation Amid Extreme Divergence in Freshwater SAR11 Hypervariable Region 2

This study characterizes freshwater SAR11 HVR2 sequences, revealing high intra-population diversity and conserved functional configurations across marine and freshwater lineages.

Novel viral diversity and geographic structuring in the New Zealand West Coast blackfly (Austrosimulium ungulatum)

This study characterizes the virome of the West Coast blackfly, identifying novel viruses and revealing geographic structuring in viral communities.

Characterization of Lactobacillus rhamnosus-Derived Extracellular Vesicles and Their Cellular Cytoprotective Effects Against Trimethylamine-Induced Oxidative Stress in Caco-2 Cells

Viral replication and host response in airway epithelial cells predicts coronavirus pathogenicity and identifies HCoV-229E as an atypical seasonal coronavirus

This study compares viral replication and host responses in airway epithelial cells to distinguish pathogenic from seasonal coronaviruses, finding HCoV-229E induces severe epithelial injury despite limited replication.

Northern palsa and peat ecosystem microbiomes are potentially supported by hydrogen and carbon monoxide metabolism

The paper characterizes microbial communities and metabolic potential in northern palsa and peat ecosystems, finding distinct microbial profiles and highlighting hydrogen and carbon monoxide metabolism as key energy sources.

Structural basis of virion assembly in the Mycobacterium tuberculosis-infecting mycobacteriophage SWU1

This study determines the atomic structure of mycobacteriophage SWU1 using cryo-EM, revealing key architectural features critical for virion assembly and infection.

Polyphosphate metabolism genes are associated with hydroxyapatite biomineralization capacity in a novel hydrogenotrophic methanogen Methanobacterium AMB_03

This study links polyphosphate metabolism genes to hydroxyapatite biomineralization in Methanobacterium AMB_03, showing that polyphosphate synthesis enables carbonate-substituted hydroxyapatite precipitation under phosphate-enriched conditions.

MmuPV1 and HPV E6 Elevate Nuclear Accumulation of IKK? to Impair NF-κB Signaling to Promote Persistent Infection

This study shows MmuPV1 and HPV16 E6 oncoproteins disrupt NF-κB signaling by increasing nuclear IKKα localization, suggesting a shared mechanism in papillomavirus persistence.

Diverse bacteria exhibit superadditive growth on mixed classes of carbon sources

The paper shows that some bacteria grow better on mixed carbon sources than on individual ones, and links this to their genetic makeup and metabolic preferences.

Cell cycle progression and stalked cell morphogenesis in Caulobacter crescentus are regulated independently by the flagellum

This study identifies fss genes that regulate stalked cell morphogenesis and cell cycle progression independently of the flagellum in Caulobacter crescentus.

41% of Gram-negative bacteria encode a Type Six Secretion System

The paper quantifies T6SS prevalence in Gram-negative bacteria using genome analysis and finds it is present in 41% of well-sampled genera.

SPARK: Systematic Profiling of Antibiotic-Phage Response Kinetics

SPARK quantifies phage-antibiotic interactions using growth curves and shows synergy, antagonism, and dynamic changes across bacterial isolates and time.

Seasonal H1N1 influenza A virus preferentially infects a pre-existing ciliogenesis-primed secretory cell state

The paper identifies a ciliogenesis-primed secretory cell state preferentially infected by seasonal H1N1 IAV in human airway epithelium.

Integrating Microbial Ecology and Sustainability: Linking Agricultural Waste Gas Streams to Microbial Community Shifts in a Photobioreactor

This study examines microbial community dynamics in a photobioreactor using agricultural waste gas streams, showing spatially structured communities and partial nitrogen recovery.

Reversible decrease in swimming motility accompanies emergent β-lactam antibiotic resistance in Escherichia coli

This study shows that reduced swimming motility accompanies B-lactam antibiotic resistance in E. coli through efflux mechanisms, with motility recovery after antibiotic removal.

Engineered DNA materials shape microbial physiology and ecological selection

This study shows engineered DNA materials influence microbial physiology and community dynamics by altering stress responses and shaping genetic interactions in wastewater microbiomes.

Environmental Frass Remodels the Larval Gut Microbiome to Enable Black Soldier Fly (Hermetia illucens) Bioconversion of Livestock Manure

This study shows that wild frass enhances BSFL growth on rabbit manure by introducing lignocellulose-degrading microbes, which are lost in captive-reared frass.