A. Nakazawa

Hypothesis: Colony-forming activity of pluripotent stem cell-derived hepatocyte-like cells for stem cell assay

The paper hypothesizes that colony-forming units in liver (CFU-L) can quantify the engraftment and functionality of hepatocyte-like cells derived from pluripotent stem cells.

Drug metabolism activity is a Critical Intrinsic Selector for Hepatocytes to elongate cell lifespan by using a cell-killing antibiotic

This study develops a method to expand human hepatocytes in vitro using a cell-killing antibiotic, enabling long-term culture and stable supply for drug research.

Puromycin selection of cells with a high expression of the cytochrome P450 CYP3A4 gene activity from a patient with drug-induced liver injury (DILI) and their lifespan prolongation using a combination of CDK4R24C, cyclin D1 and TERT

The paper generates an immortalized hepatocyte cell line with high CYP3A4 expression from a DILI patient and shows its prolonged lifespan and drug-metabolism capacity.

Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

This study shows that ammonia treatment enriches hepatocyte-like cells derived from human pluripotent stem cells, which can be propagated long-term in vitro while maintaining liver-specific gene expression.

Immortalization of human zone I hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing

The paper describes the creation of an immortalized hepatocyte cell line, HepaMN, from biliary atresia tissue and demonstrates its utility for cytochrome P450 induction testing.

A survey of public attitudes towards third-party reproduction in Japan in 2014

This study surveys public attitudes towards third-party reproduction and disclosure in Japan, finding that gender, age, and infertility experience influence opinions.