A. Umezawa

Human intestinal organoid-derived PDGFRα+ mesenchymal stroma empowers LGR4+ epithelial stem cells

This study shows that organoid-derived mesenchymal stromal cells support the proliferation and functionality of hPSC-derived small intestinal epithelial stem cells in adherent culture.

Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

This study generates functional and long-lived melanocytes from human pluripotent stem cells using transient JMJD3 expression, showing melanogenesis and engraftability in mice.

Drug metabolic activity as a selection factor for pluripotent stem cell-derived hepatic progenitor cells

This study isolates and expands hepatic progenitor cells from human ESCs by selecting for drug-metabolizing activity, enabling a stable supply of functional liver cells for therapy and drug testing.

Novel therapeutic strategies for injured endometrium: Autologous intrauterine transplantation of menstrual blood-derived cells from infertile patients

This study evaluates the therapeutic potential of menstrual blood-derived cells from infertile patients for injured endometrium, showing improved endometrial thickness and fertility outcomes in a mouse model.

Hypothesis: Colony-forming activity of pluripotent stem cell-derived hepatocyte-like cells for stem cell assay

The paper hypothesizes that colony-forming units in liver (CFU-L) can quantify the engraftment and functionality of hepatocyte-like cells derived from pluripotent stem cells.

Drug metabolism activity is a Critical Intrinsic Selector for Hepatocytes to elongate cell lifespan by using a cell-killing antibiotic

This study develops a method to expand human hepatocytes in vitro using a cell-killing antibiotic, enabling long-term culture and stable supply for drug research.

Characterization of human embryonic stem cells in animal component-free medium

This study shows that SEES2 ESCs can be stably cultured in an animal component-free medium for 60 days without losing their karyotype or differentiation potential.

Gorlin syndrome-induced pluripotent stem cells form medulloblastoma with loss of heterozygosity in PTCH1

This study shows that Gorlin syndrome patient-derived iPSCs form medulloblastoma in mice, with one tumor showing PTCH1 loss of heterozygosity.

Puromycin selection of cells with a high expression of the cytochrome P450 CYP3A4 gene activity from a patient with drug-induced liver injury (DILI) and their lifespan prolongation using a combination of CDK4R24C, cyclin D1 and TERT

The paper generates an immortalized hepatocyte cell line with high CYP3A4 expression from a DILI patient and shows its prolonged lifespan and drug-metabolism capacity.

Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

This study shows that ammonia treatment enriches hepatocyte-like cells derived from human pluripotent stem cells, which can be propagated long-term in vitro while maintaining liver-specific gene expression.

Immortalization of human zone I hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing

The paper describes the creation of an immortalized hepatocyte cell line, HepaMN, from biliary atresia tissue and demonstrates its utility for cytochrome P450 induction testing.

Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors

The paper compares epigenetic differences in human iPSCs generated by three different vectors and finds Sendai virus generates the fewest aberrant methylation sites.

Can Human Embryonic Stem Cell-Derived Stromal Cells Serve A Starting Material For Myoblasts?

This study generates ESC-derived CD105+ stromal cells with long-term proliferation and myogenic potential as a cell source for myoblast therapy in muscular disorders.

Fetal therapy model of myelomeningocele with three-dimensional skin using amniotic fluid cell-derived induced pluripotent stem cells

This study uses iPSC-derived artificial skin to treat a rat model of myelomeningocele during pregnancy, showing potential for antenatal therapy.