Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity
D. Hoving, A. H. C. Marques, W. Huisman, B. A. Nosoh, A. C. de Kroon, O. R. J. van Hengel, B. R. Wu, P. M. van Helden, B. C. Urban, N. Dhar, D. M. Ferreira, G. Kwatra, C. H. Hokke, S. P. Jochems
This study develops a high-throughput method to analyze polysaccharide-specific B cells using combinatorial multimer staining and spectral flow cytometry, revealing associations with serotype specificity, vaccination history, and geographic origin.
bioRxiv · Mar 7, 2023Immunology