Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics
Kathrin Leppek, Gun Woo Byeon, Wipapat Kladwang, Hannah K. Wayment-Steele, Craig H. Kerr, Adele Xu, Do Soon Kim, Ved V. Topkar, Christian A. Choe, Daphna Rothschild, Gerald C. Tiu, Roger Wellington-Oguri, Kotaro Fujii, Eesha Sharma, Andrew M. Watkins, , Rhiju Das
This study develops PERSIST-seq and In-line-seq to optimize mRNA stability and translation, finding that in-cell stability is more critical than ribosome load for protein output.
Nature Communications · Mar 22, 2022Peer-reviewedMolecular Biology