The paper designs and selects ribosomal active site mutants with high activity using a computational approach to overcome limitations in rRNA modification.

bioRxiv · Jun 2, 2022 · PreprintSynthetic Biology

Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics

Kathrin Leppek, Gun Woo Byeon, Wipapat Kladwang, Hannah K. Wayment-Steele, Craig H. Kerr, Adele Xu, Do Soon Kim, Ved V. Topkar, Christian A. Choe, Daphna Rothschild, Gerald C. Tiu, Roger Wellington-Oguri, Kotaro Fujii, Eesha Sharma, Andrew M. Watkins, , Rhiju Das

This study develops PERSIST-seq and In-line-seq to optimize mRNA stability and translation, finding that in-cell stability is more critical than ribosome load for protein output.

Nature Communications · Mar 22, 2022Peer-reviewedMolecular Biology

In vitro ribosome synthesis and evolution through ribosome display

Michael J. Hammerling, Brian R. Fritz, Danielle J. Yoesep, Do Soon Kim, Erik D. Carlson, Michael C. Jewett

This study develops an in vitro method for ribosome synthesis and evolution called RISE, demonstrating its ability to select for ribosome variants resistant to clindamycin and revealing positive epistasis in successful genotypes.

Nature Communications · Feb 28, 2020Peer-reviewedSynthetic Biology