Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope
Steven Milan Moreno, Sharika Mohanan, Ahmed Elnageh, Erin Boland, Lewis Williamson, Camilla Olianti, Leonardo Sacconi, Godfrey L. Smith, Eline Huethorst, Marie Caroline Müllenbroich
The paper introduces a no-shear imaging protocol for mesoSPIM light-sheet microscopy to improve imaging of thin tissue slices with reduced processing time and better axial resolution.
Biomedical Optics Express · Dec 23, 2025Peer-reviewedBiophysics