Author

J. B. Hein

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Biochemistry· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:protein folding & dynamics· 2 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Assay:cryo-EM· 1 Research Focus:development & morphogenesis· 1 Research Focus:drug response / drug discovery· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

P. M. Fordyce· 3 shared J. Nilsson· 2 shared Y. Feng· 2 shared A. K. White· 1 shared A. Kettenbach· 1 shared B. Lopez-Mendez· 1 shared D. Garvanska· 1 shared E. Appel· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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3 shared papersBioengineering

P. M. Fordyce

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19

S. K. Padi, M. R. Vos, R. J. Godek, J. R. Fuller, T. Kruse, J. B. Hein, J. Nilsson, M. S. Kelker, R. Page, W. Peti

The paper reports cryo-EM structures of PP2A:B55 bound to phosphorylated ARPP19 and FAM122A, revealing distinct binding mechanisms and providing insights for therapeutic development.

bioRxiv · Aug 31, 2023Biochemistry

Global substrate identification and high throughput in vitro dephosphorylation reactions uncover PP1 and PP2A-B55 specificity principles

J. B. Hein, H. T. Nguyen, D. Garvanska, I. Nasa, Y. Feng, B. Lopez-Mendez, N. E. Davey, A. Kettenbach, P. M. Fordyce, J. Nilsson

The paper develops an in vitro assay to determine phosphatase specificity and applies it to study mitotic exit, revealing substrate preferences and biological relevance.

bioRxiv · May 14, 2023 · PreprintCell Biology

MRBLE-pep measurements reveal accurate binding affinities for B56, a PP2A regulatory subunit

J. B. Hein, M. S. Cyert, P. M. Fordyce

The paper compares MRBLE-pep and ITC measurements of B56 binding affinities, showing MRBLE-pep accurately quantifies relative affinity with less material.

ACS Measurement Science Au · Oct 20, 2021Peer-reviewedBioengineering

MRBLEs 2.0: High-throughput generation of chemically functionalized spectrally and magnetically-encoded hydrogel beads using a simple single-layer microfluidic device

Y. Feng, A. K. White, J. B. Hein, E. Appel, P. M. Fordyce

The paper introduces MRBLEs 2.0, a method for high-throughput synthesis of functionalized microspheres with spectral and magnetic encoding, demonstrating efficient conjugation of biomolecules and magnetic nanoparticle incorporation.

bioRxiv · Jun 22, 2020Bioengineering