Author

J. G. Skeate

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Bioengineering· 3

Signature Assays, Organisms & Methods

Click to filter · or open tag

Organism:human· 3 Research Focus:cell type annotation· 2 Assay:CRISPR screen· 2 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Approach:genetic perturbation / CRISPR· 2 Approach:deep learning· 1 Approach:experimental assay· 1

Frequent Co-Authors

B. R. Webber· 3 shared B. S. Moriarity· 3 shared W. S. Lahr· 3 shared E. J. Pomeroy· 2 shared J. B. Krueger· 2 shared N. J. Slipek· 2 shared A. K. Gilkey· 1 shared A. P. DeFeo· 1 shared

Activity by Year

2021-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 34 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersBioengineering

B. R. Webber

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Precision Enhancement of CAR-NK Cells through Non-Viral Engineering and Highly Multiplexed Base Editing

M. Wang, J. B. Krueger, A. K. Gilkey, E. M. Stelljes, M. G. Kluesner, E. J. Pomeroy, J. G. Skeate, N. J. Slipek, W. S. Lahr, P. N. Claudio Vazquez, Y. Zhao, E. J. Eaton, K. Laoharawee, B. R. Webber, B. S. Moriarity

This study enhances CAR-NK cell functionality using non-viral base editing and transposon integration, showing improved therapeutic potential in Burkitt's lymphoma models.

bioRxiv · Mar 5, 2024Bioengineering

Homology mediated end joining enables efficient non-viral targeted integration of large DNA templates in primary human T cells

B. R. Webber, M. J. Johnson, N. S. Slipek, W. S. Lahr, A. P. DeFeo, J. G. Skeate, X. Qiu, B. Rathmann, M. D. Diers, B. Wick, T. Henley, M. Choudhry, R. S. McIvor, B. S. Moriarity

This paper describes a non-viral method for efficiently integrating large DNA into primary human T cells using CRISPR-Cas9 and homology-mediated end joining, achieving high functionality and anti-tumor activity.

bioRxiv · Nov 12, 2021 · PreprintBioengineering

Non-Viral Engineering of CAR-NK and CAR-T cells using the Tc Buster Transposon System™

E. J. Pomeroy, W. S. Lahr, J. W. Chang, J. B. Krueger, B. J. Wick, N. J. Slipek, J. G. Skeate, B. R. Webber, B. S. Moriarity

This paper demonstrates a non-viral method using the Tc Buster transposon system to deliver CAR constructs to primary human NK and T cells, achieving efficient gene expression and functional activity.

bioRxiv · Aug 2, 2021 · PreprintBioengineering