Highly specific chimeric DNA-RNA guided genome editing with enhanced CRISPR-Cas12a system
H. Kim, W.-j. Lee, C. H. Kim, Y. Oh, L. W. Gwon, H. Lee, W. Song, J. K. Hur, K.-S. Lim, K. J. Jeong, K.-H. Nam, Y.-S. Won, Y. Lee, Y.-H. Kim, J.-W. Huh, , S. H. Lee
The paper enhances CRISPR-Cas12a system specificity and efficiency using a chimeric DNA-RNA guide, achieving 7.41 and 7.60 times improvements respectively.
bioRxiv · Sep 4, 2021Biochemistry