Author

John A. Walker

Synthego (United States)

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Developmental Biology· 1 Genetics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:CRISPR screen· 1 Research Focus:differential expression· 1 Research Focus:gene regulatory network inference· 1 Organism:mouse· 1 Research Focus:perturbation response prediction· 1 Research Focus:variant calling· 1 Organism:zebrafish· 1

Frequent Co-Authors

Kevin HoldeniD· 2 shared Alejandra Cano-RuiziD· 1 shared Alejandro Díaz‐MoscosoiD· 1 shared Amr R. Ghanam· 1 shared Anastasia P. Kadina· 1 shared Anoja G. PereraiD· 1 shared Anthony J. TreicheliD· 1 shared Ariel BazziniiD· 1 shared

Activity by Year

2021-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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2 shared papersDevelopmental Biology

Kevin HoldenORCID

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Author profile →

2 papers

Recent·Most cited

Enhanced RNA-targeting CRISPR-Cas technology in zebrafish

Ismael Moreno-Sánchez, Luis Hernández-Huertas, Daniel Nahón-Cano, Pedro Manuel Martínez‐García, Anthony J. Treichel, Carlos Gómez-Marín, Laura Tomás‐Gallardo, Gabriel da Silva Pescador, Gopal Kushawah, Rhonda Egidy, Anoja G. Perera, Alejandro Díaz‐Moscoso, Alejandra Cano-Ruiz, John A. Walker, Manuel J. Muñoz, , Miguel A. Moreno-Mateos

This study optimizes CRISPR-Cas13d for RNA targeting in zebrafish by improving gRNA design, nuclear targeting, and computational prediction, reducing collateral effects.

Nature Communications · Mar 16, 2025Peer-reviewedDevelopmental Biology

Prime Editing in Mice Reveals the Essentiality of a Single Base in Driving Tissue-Specific Gene Expression

Pan Gao, Qing Lyu, Amr R. Ghanam, Cícera Regina Lazzarotto, Gregory A. Newby, Wei Zhang, Mi‐Hyun Choi, Orazio J. Slivano, Kevin Holden, John A. Walker, Anastasia P. Kadina, Rob J. Munroe, Christian M. Abratte, John C. Schimenti, David R. Liu, , Joseph M. Miano

This paper compares CRISPR-mediated HDR and PE2 systems in mice to show PE2's higher fidelity in editing a TFBS, leading to more effective tissue-specific gene inactivation.

Genome biology · Mar 15, 2021Peer-reviewedGenetics