Author

K. Alagesan

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Assay:proteomics· 4 Research Focus:cell type annotation· 3 Organism:human· 3 Research Focus:protein folding & dynamics· 3 Research Focus:clustering· 2 Assay:metabolomics· 2 Approach:benchmark study· 1

Frequent Co-Authors

D. Kolarich· 4 shared D. Varon Silva· 2 shared P. H. Seeberger· 2 shared A. Chernykh· 1 shared A. Everest-Dass· 1 shared A. Pap· 1 shared A. Ruda· 1 shared B. L. Parker· 1 shared

Activity by Year

2019-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 15 mutual co-author links

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4 shared papersBiochemistry

D. Kolarich

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines

S. Ajay Castro, I. J. Passmore, D. Ndeh, H. A. Shaw, A. Ruda, K. Burns, S. Thomson, R. Nagar, K. Alagesan, K. Lucas, S. Abouelhadid, M. Reglinski, U. Schwarz- Linek, F. Mawas, G. Widmalm, , H. C. Dorfmueller

This study develops a platform for recombinant production of Group A Streptococcus glycoconjugate vaccines using E. coli, confirming their structural integrity and immunogenicity in animal models.

bioRxiv · Mar 1, 2024Microbiology

IMPROVED N- AND O-GLYCOPEPTIDE IDENTIFICATION USING HIGH-FIELD ASYMMETRIC WAVEFORM ION MOBILITY SPECTROMETRY (FAIMS)

K. Alagesan, R. Ahmed-Begrich, E. Charpentier

This study shows that FAIMS improves detection of N- and O-glycopeptides without enrichment, increasing detection by 31% compared to standard methods.

bioRxiv · Dec 12, 2022 · PreprintBiochemistry

Better together - Relative retention time plus spectral matching improves automated glycan characterization using PGC-nLC-IT-ESI-MS/MS

K. Alagesan, F. Schirmeister, U. Moeginger, A. Everest-Dass, F. Altmann, P. H. Seeberger, M. von Itzstein, N. H. Packer, D. Kolarich

The paper introduces GlycoRRT, a tool combining retention time and spectral analysis for automated glycan characterization using PGC-nLC-MS/MS, and applies it to human and animal glycomics data.

bioRxiv · Aug 4, 2021 · PreprintBiochemistry

Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data

R. Kawahara, A. Chernykh, K. Alagesan, M. Bern, W. Cao, R. J. Chalkley, K. Cheng, M. S. Choo, N. Edwards, R. Goldman, M. Hoffmann, Y. Hu, Y. Huang, J. Y. Kim, D. Kletter, , Palmisano

This study evaluates glycoproteomics software performance using human serum datasets, identifies high-performance strategies, and provides recommendations for improved analysis.

bioRxiv · Mar 14, 2021 · PreprintBiochemistry

A novel, ultrasensitive approach for quantitative carbohydrate composition and linkage analysis using LC-ESI ion trap tandem mass spectrometry

K. Alagesan, D. Varon Silva, P. H. Seeberger, D. Kolarich

This paper introduces a sensitive method for quantifying and analyzing carbohydrate composition and linkages using LC-ESI-MS/MS with permethylation and reductive amination steps.

bioRxiv · Nov 24, 2019 · PreprintBiochemistry

Glycan size and attachment site location affect electron transfer dissociation (ETD) fragmentation and automated glycopeptide identification

K. Alagesan, H. Hinneburg, P. Seeberger, D. Varon Silva, D. Kolarich

The paper evaluates how glycan size and attachment site location affect ETD fragmentation and automated glycopeptide identification using a synthetic glycopeptide library.

bioRxiv · Jun 24, 2019Biochemistry

To enrich or not to enrich: Enhancing (glyco)peptide ionization using the CaptiveSpray nanoBooster ™

K. Alagesan, D. Kolarich

This study evaluates dopant solvents to enhance glycopeptide ionization using the CaptiveSpray nanoBooster, finding acetonitrile provides the best signal increase without enrichment.

bioRxiv · Apr 5, 2019 · PreprintBiochemistry