Author

K. H. Gardner

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Biophysics· 3

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:protein folding & dynamics· 3 Assay:protein structure· 3 Research Focus:cell signaling & trafficking· 2 Assay:proteomics· 2 Approach:wet-lab / biochemical assay· 2 Research Focus:development & morphogenesis· 1 Research Focus:drug response / drug discovery· 1

Frequent Co-Authors

J. M. Aramini· 3 shared U. R. Edupuganti· 3 shared X. Xu· 3 shared D. C. Favaro· 2 shared D. Gagne· 2 shared L. P. Marcelino· 2 shared R. Azad· 2 shared A. A. Gil· 1 shared

Activity by Year

2020-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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3 shared papersBiophysics

J. M. Aramini

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Author profile →

6 papers

Recent·Most cited

Identification of Small Molecule Ligand Binding Sites On and In the ARNT PAS-B Domain

X. Xu, J. Closson, L. P. Marcelino, D. C. Favaro, M. L. Silvestrini, R. Solazzo, L. T. Chong, K. H. Gardner

The paper identifies ligand-binding sites on and within the ARNT PAS-B domain using NMR, MD simulations, and ensemble docking, revealing distinct binding modes for three small molecules.

bioRxiv · Nov 3, 2023Biochemistry

Signal-regulated unmasking of the nuclear localization motif in the PAS domain regulates the nuclear translocation of PASK.

M. Xiao, S. Dhungel, R. Azad, D. C. Favaro, R. R. Pushpabai, K. H. Gardner, C. KIKANI

This study identifies a mechanism by which PASK's nuclear translocation is regulated by the interaction between its PAS-A domain and a PIM motif, which is modulated by metabolic signals.

bioRxiv · Sep 6, 2023Biochemistry

Use of High Pressure NMR Spectroscopy to Rapidly Identify Proteins with Internal Ligand-Binding Voids

D. Gagne, R. Azad, J. M. Aramini, X. Xu, E. A. Isiorho, U. R. Edupuganti, J. P. Williams, L. P. Marcelino, B. A. Johnson, K. Akasaka, K. H. Gardner

This paper introduces a method using high-pressure NMR spectroscopy to identify proteins with internal ligand-binding voids by detecting nonlinear chemical shift changes in 1H/15N HSQC experiments.

bioRxiv · Aug 25, 2020 · PreprintBiophysics

High-Pressure NMR Reveals Volume and Compressibility Differences Between Two Stably Folded Protein Conformations

X. Xu, D. Gagne, J. M. Aramini, K. H. Gardner

The paper uses high-pressure NMR to study conformational changes in ARNT PAS-B Y456T, finding that the slipped conformation is smaller and less compressible than the wildtype and involves an unfolded intermediate.

bioRxiv · Jul 29, 2020Biophysics

Diversity of function and higher-order structure within HWE sensor histidine kinases

I. Dikiy, D. Swingle, K. Toy, U. R. Edupuganti, G. Rivera-Cancel, K. H. Gardner

The paper examines the diversity of oligomeric states and regulatory mechanisms in HWE/HisKA2 histidine kinases, identifying monomeric and dimeric forms with distinct functional responses.

bioRxiv · Jul 8, 2020Biochemistry

Unraveling the Mechanism of a LOV Domain Optogenetic Sensor: A Glutamine Lever Induces Unfolding of the Jα Helix

J. N. Iuliano, J. Tolentino Collado, A. A. Gil, P. V. Ravindran, A. Lukacs, S. Shin, H. A. Woroniecka, K. Adamczyk, J. M. Aramini, U. R. Edupuganti, C. R. Hall, G. M. Greetham, I. V. Sazanovich, I. P. Clark, T. Daryaee, , P. J. Tonge

This study investigates the structural dynamics of the LOV2 domain in AsLOV2, showing that Q513 and N414 mediate J-helix unfolding linked to optogenetic function.

bioRxiv · Jan 29, 2020Biophysics