K. W. Leong

Human vascular organoids with a mosaic AKT1 mutation recapitulate Proteus syndrome

This paper creates a vascular organoid model with an AKT1 mutation to study Proteus syndrome and finds AKT inhibitors reverse vascular malformations.

Focused ultrasound-mediated brain genome editing

This paper demonstrates a method to edit the brain genome in mammals using focused ultrasound to temporarily open the blood-brain barrier for CRISPR/Cas9 delivery via intravenous injection.

Targeted Protein Degradation via Nanoparticles

This paper introduces a generic nanoparticle-based method for targeted protein degradation that avoids case-by-case design and enables degradation of both intracellular and extracellular targets.

An Immune Cell Recirculation-Enabled Microfluidic Array to Study Dynamic Immunotherapeutic Activity in Recapitulated Tumor Microenvironment

The paper develops a microfluidic array to study immune cell interactions in a tumor microenvironment, showing that anti-PD-L1 treatment improves T cell infiltration and reduces cancer cell apoptosis.

Mapping morphological malformation to genetic dysfunction in blood vessel organoids with 22q11.2 Deletion Syndrome

This study uses blood vessel organoids from 22q11.2 DS patients to link genetic dysfunction to vascular malformations through single-cell transcriptomics and imaging.