Kaspar Valgepea

Targeted Deletions Uncover Previously Uncharacterised C1-fixing Cluster Genes as Essential for Autotrophy in Clostridium autoethanogenum

The paper identifies essential C1-fixing cluster genes in Clostridium autoethanogenum using gene deletions and functional analysis, revealing their roles in autotrophy and metabolism.

Gas uptake stoichiometry governs carbon partitioning in syngas-fermenting Clostridium autoethanogenum

This study quantifies how syngas composition affects growth and metabolite production in Clostridium autoethanogenum during fermentation.

Genetic engineering of carbon monoxide dehydrogenases produces distinct autotrophic phenotypes in Clostridium autoethanogenum

This study genetically modifies CODH enzymes in Clostridium autoethanogenum to alter autotrophic phenotypes, revealing distinct metabolic and growth effects.

Measure Catabolism: Real-time shifts in microbial metabolism through online pressure measurements

This paper introduces a method to measure microbial catabolism in real-time using pressure measurements, distinguishing metabolic shifts not detectable by biomass alone.

Systems-level analysis identifies protein-RNA interactions contributing to autotrophy of Clostridium autoethanogenum

The paper identifies genome-wide protein-RNA interactions in Clostridium autoethanogenum that contribute to autotrophy using a combination of experimental and computational methods.

Different growth pattern during microbial electrosynthesis using C. ljungdahlii evolutionary adapted on iron

This study evolved Clostridium ljungdahlii on iron to improve extracellular electron uptake, finding reduced growth on H2 and CO2 but increased acetate production on iron compared to wild-type.

Heterologous expression of pyruvate formate lyase enhances cell growth of Clostridium ljungdahlii during microbial electrosynthesis

This study enhances cell growth of Clostridium ljungdahlii during microbial electrosynthesis by heterologously expressing pyruvate formate lyase genes from Acetobacterium woodii.

Deletion of genes linked to the C1-fixing gene cluster affects growth, by-products, and proteome of Clostridium autoethanogenum

This study investigates the effects of deleting two genes linked to the C1-fixing gene cluster in Clostridium autoethanogenum, revealing growth defects and altered by-product profiles.

RNA-seq sample preparation kits strongly affect transcriptome profiles of a gas-fermenting bacterium

This paper compares RNA-seq library preparation kits for a gas-fermenting bacterium, finding significant differences in transcriptome profiles and highlighting the importance of selecting appropriate kits for accurate results.

Faster growth enhances low carbon fuel and chemical production through gas fermentation

This study shows that faster growth of Clostridium autoethanogenum improves gas fermentation yields and productivities for ethanol and 2,3-butanediol.

Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum

This study quantifies the proteome of Clostridium autoethanogenum under different gas conditions, revealing proteome allocation priorities and enzyme dynamics.

Engineering and Evolution of Methanol Assimilation in Saccharomyces cerevisiae

This paper engineers methanol assimilation pathways in yeast and identifies a latent methylotrophic metabolism, improving growth and metabolite labeling through genetic modifications.