Michael Köpke

This study investigates the effects of deleting two genes linked to the C1-fixing gene cluster in Clostridium autoethanogenum, revealing growth defects and altered by-product profiles.

This paper compares RNA-seq library preparation kits for a gas-fermenting bacterium, finding significant differences in transcriptome profiles and highlighting the importance of selecting appropriate kits for accurate results.

The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.

This study shows that faster growth of Clostridium autoethanogenum improves gas fermentation yields and productivities for ethanol and 2,3-butanediol.

This study quantifies the proteome of Clostridium autoethanogenum under different gas conditions, revealing proteome allocation priorities and enzyme dynamics.

Spacer2PAM predicts functional PAM sequences for endogenous CRISPR systems, enabling targeted genome modification in difficult-to-transform organisms.

This paper develops methods to apply transposon-insertion sequencing in Clostridium autoethanogenum to identify essential genes for growth using carbon monoxide.

This paper develops a high-yielding cell-free gene expression system from Clostridium autoethanogenum to enable in vitro prototyping of genetic parts and metabolic pathways.

The paper introduces iPROBE, a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes, demonstrating improved production of 3-hydroxybutyrate and n-butanol in microbial cell factories.

This paper develops modular plasmids for cell-free expression and genetic assembly in Clostridium to accelerate biosynthetic pathway testing.