Séan Dennis Simpson

The paper optimizes and implements reverse beta-oxidation pathways in bacteria for carbon-negative synthesis of C4-C6 acids and alcohols, achieving high product titers in engineered strains.

This study quantifies the proteome of Clostridium autoethanogenum under different gas conditions, revealing proteome allocation priorities and enzyme dynamics.

This paper develops methods to apply transposon-insertion sequencing in Clostridium autoethanogenum to identify essential genes for growth using carbon monoxide.

This paper develops a high-yielding cell-free gene expression system from Clostridium autoethanogenum to enable in vitro prototyping of genetic parts and metabolic pathways.

The paper introduces iPROBE, a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes, demonstrating improved production of 3-hydroxybutyrate and n-butanol in microbial cell factories.

This paper develops modular plasmids for cell-free expression and genetic assembly in Clostridium to accelerate biosynthetic pathway testing.