L. M. Blank

Establishing a straight-forward I-SceI mediated recombination one plasmid system for efficient genome editing in P. putida KT2440

The paper introduces a one-plasmid genome editing system for P. putida KT2440, enabling rapid and efficient genome modifications in three days.

Bioprocess optimization enables enhanced protopanaxadiol production in yeast

This study optimizes bioprocess conditions to enhance protopanaxadiol production in yeast using Design of Experiment, achieving a 7.5-fold increase in yield.

Itaconic acid production by co-feeding of Ustilago maydis: a combined approach of experimental data, design of experiments and metabolic modeling

This study optimizes itaconic acid production by Ustilago maydis through co-feeding glucose and acetate, combining experimental data, design of experiments, and metabolic modeling.

LC-UV/RI-MS2 as the analytical platform for bioconversion of sustainable carbon sources: a showcase of 1,4-butanediol plastic monomer degradation using Ustilago trichophora

This paper develops an LC-UV/RI-MS2 method to analyze metabolites secreted by Ustilago trichophora during BDO degradation, identifying malic acid, succinic acid, erythritol, and mannitol with 33% carbon yield.

Genomic and metabolic plasticity drive alternative scenarios for adapting Pseudomonas putida to non-native substrate D-xylose.

This study investigates how Pseudomonas putida adapts to xylose by combining metabolic engineering and adaptive evolution, revealing genomic and metabolic plasticity that enables growth on the non-native sugar.

Evaluating microbial contaminations of alternative heating oils

This study evaluates microbial contamination risks of alternative heating oils using CO2 monitoring and simulation, finding that some fuels resist contamination while others promote microbial growth.

Triterpenoid production with a minimally engineered Saccharomyces cerevisiae chassis

This paper engineers Saccharomyces cerevisiae to produce triterpenoids at high titers by modifying an oxidosqualene cyclase, demonstrating a chassis for diverse triterpenoid synthesis.

Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

This study identifies genes and proteins involved in nitrogen metabolism in Pseudomonas putida using transposon sequencing and provides an interactive visualization tool for the data.

Biotechnology data analysis training with Jupyter Notebooks

This paper develops a virtual organism simulator and Jupyter Notebook-based instructional workflow to teach biotechnology data analysis through simulated experiments.

Functional analysis of the fatty acid and alcohol metabolism of Pseudomonas putida using RB-TnSeq

This study uses RB-TnSeq to functionally analyze fatty acid and alcohol metabolism in Pseudomonas putida, identifying gene roles and catabolic pathways for isoprenol and isopentanol.

Bio-upcycling of polyethylene terephthalate

This paper presents a novel method to convert polyethylene terephthalate into two bioplastics using enzymatic hydrolysis and microbial fermentation.

Insights into an alternative pathway for glycerol metabolism in a glycerol kinase deficient Pseudomonas putida KT2440

This study identifies an alternative glycerol metabolism pathway in a glycerol kinase-deficient Pseudomonas putida KT2440 strain, revealing upregulated genes and a new enzymatic function for 3-hydroxybutyrate dehydrogenase.