Author

M. Fukuyama

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 3 Research Focus:cell type annotation· 2 Research Focus:gene regulatory network inference· 2 Organism:C. elegans· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:clustering· 1 Assay:CRISPR screen· 1

Frequent Co-Authors

D. Kitagawa· 3 shared S. Hata· 3 shared T. Chinen· 3 shared A. Mabuchi· 2 shared M. Genova· 2 shared T. Harada· 2 shared T. Komori· 2 shared A. Kaech· 1 shared

Activity by Year

2019-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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3 shared papersCell Biology

D. Kitagawa

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4 papers

Recent·Most cited

An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging

T. Harada, S. Hata, M. Fukuyama, T. Chinen, D. Kitagawa

This study identifies ascorbic acid as an effective antioxidant to prevent light-induced mitotic defects in live cell imaging of human diploid cells.

Communications Biology · Nov 1, 2023Peer-reviewedCell Biology

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid cells

A. Mabuchi, S. Hata, M. Genova, C. Tei, K. K. Ito, M. Hirota, T. Komori, M. Fukuyama, T. Chinen, A. Toyoda, D. Kitagawa

This study compares ssDNA and dsDNA as HDR donors for CRISPR-mediated gene tagging in human diploid cells, finding dsDNA more efficient for precise insertion.

bioRxiv · Jun 1, 2022 · PreprintCell Biology

A CRISPR-del-based pipeline for complete gene knockout in human diploid cells

T. Komori, S. Hata, A. Mabuchi, M. Genova, T. Harada, M. Fukuyama, T. Chinen, D. Kitagawa

This study introduces a CRISPR-del pipeline to achieve complete gene knockout in human diploid cells by inducing large chromosomal deletions, showing higher efficiency than conventional methods.

bioRxiv · Jun 21, 2021Cell Biology

Patched regulates lipid homeostasis by controlling cellular cholesterol levels

C. E. Cadena Del Castillo, J. T. Hannich, A. Kaech, H. Chiyoda, M. Fukuyama, N. J. Færgeman, H. Riezman, A. Spang

This study identifies PTC-3 as a cholesterol transporter in C. elegans, showing that its loss causes cholesterol accumulation, ER defects, and lipid metabolism issues, which are rescued by reducing dietary cholesterol.

bioRxiv · Oct 23, 2019Cell Biology