Author

M. Hedglin

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 7

Signature Assays, Organisms & Methods

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Research Focus:chromatin & epigenetic regulation· 7 Approach:experimental assay· 7 Organism:human· 5 Research Focus:cell signaling & trafficking· 4 Assay:cryo-EM· 2 Assay:DNA methylation· 2 Research Focus:protein structure prediction· 2 Research Focus:differential expression· 1

Frequent Co-Authors

K. G. Pytko· 3 shared R. L. Dannenberg· 3 shared E. Antony· 2 shared V. Kaushik· 2 shared A. Al-Amodi· 1 shared A. De Biasio· 1 shared A. U. Danazumi· 1 shared B. A. Kelch· 1 shared

Activity by Year

2020-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 11 mutual co-author links

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3 shared papersBiochemistry

K. G. Pytko

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7 papers

Recent·Most cited

Structure of the human CTF18-RFC clamp loader bound to PCNA

G. Briola, M. Tehseen, A. Al-Amodi, G. Young, A. U. Danazumi, P. Q. Nguyen, C. G. Savva, M. Hedglin, S. M. Hamdan, A. De Biasio

This study presents a cryo-EM structure of the human CTF18-RFC complex bound to PCNA, revealing structural features important for PCNA loading and Pol ε-mediated leading strand synthesis.

bioRxiv · May 8, 2024 · PreprintBiochemistry

Replication of 25 microsatellite sequences by human DNA polymerase delta holoenzymes is dependent on dNTP and RPA levels

K. G. Pytko, R. L. Dannenberg, K. A. Eckert, M. Hedglin

This study investigates how human DNA polymerase delta holoenzymes replicate [AT/TA]25 microsatellite sequences and finds that replication efficiency depends on dNTP and RPA levels.

bioRxiv · Nov 7, 2023Biochemistry

Interplay of macromolecular interactions during assembly of human DNA polymerase δ holoenzymes and initiation of DNA synthesis

J. L. Norris, L. O. Rogers, K. G. Pytko, R. L. Dannenberg, S. Perreault, V. Kaushik, S. Kuppa, E. Antony, M. Hedglin

This study uses FRET assays to show how RPA interactions at P/T junctions facilitate Pol δ holoenzyme assembly and DNA synthesis efficiency.

bioRxiv · May 9, 2023 · PreprintBiochemistry

Mechanistic Insight into AP-Endonuclease 1 Cleavage of Abasic Sites at Stalled Replication Forks

N. M. Hoitsma, J. Norris, T. Khoang, V. Kaushik, E. Antony, M. Hedglin, B. Freudenthal

This study investigates APE1 cleavage of abasic sites at replication forks, revealing its mechanism and interaction with RPA.

bioRxiv · Aug 22, 2022 · PreprintBiochemistry

High resolution studies of DNA lesion bypass by human DNA polymerase δ holoenzymes

R. L. Dannenberg, J. A. Cardina, K. G. Pytko, M. Hedglin

This study quantitatively examines how human DNA polymerase δ holoenzymes handle DNA lesions during replication, showing they can replicate lesions and their effects depend on lesion type and proofreading.

bioRxiv · Jun 30, 2022Biochemistry

PCNA monoubiquitination is regulated by diffusion of Rad6/Rad18 complexes along RPA filaments

M. Li, B. Sengupta, S. J. Benkovic, T. H. Lee, M. Hedglin

The paper investigates how Rad6/Rad18 complexes move along RPA filaments to regulate PCNA monoubiquitination during translesion DNA synthesis.

bioRxiv · Aug 11, 2020Biochemistry

Structure of the human clamp loader bound to the sliding clamp: a further twist on AAA+ mechanism

C. Gaubitz, X. Liu, J. Magrino, N. P. Stone, J. Landeck, M. Hedglin, B. A. Kelch

This paper reports the cryo-EM structure of human RFC bound to PCNA, revealing an autoinhibited conformation and a novel regulatory mechanism for AAA+ ATPases.

bioRxiv · Feb 18, 2020Biochemistry