V. Kaushik

Wrapping of single-stranded DNA by Replication Protein A and modulation through phosphorylation

This study uses single-molecule techniques to show that RPA partially wraps ssDNA and that phosphorylation at Ser-384 modulates this wrapping.

Multiplexed screen identifies a Pseudomonas aeruginosa-specific small molecule targeting the outer membrane protein OprH and its interaction with LPS

The paper identifies a Pseudomonas aeruginosa-specific small molecule, BRD1401, that targets OprH and disrupts its interaction with LPS, increasing membrane fluidity.

Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.

Fluorescent human RPA to track assembly dynamics on DNA

This paper describes fluorescently modified human RPA to study its dynamic interactions with ssDNA and other proteins.

Interplay of macromolecular interactions during assembly of human DNA polymerase δ holoenzymes and initiation of DNA synthesis

This study uses FRET assays to show how RPA interactions at P/T junctions facilitate Pol δ holoenzyme assembly and DNA synthesis efficiency.

Human hnRNPA1 reorganizes telomere-bound Replication Protein A

The paper shows hnRNPA1 remodels RPA bound to telomeric ssDNA, forming a ternary complex regulated by TERRA.

An Aurora B-RPA signaling axis secures chromosome segregation fidelity

This study identifies a mitosis-specific regulation of RPA by Aurora B kinase, showing that phosphorylation of RPA70 at Ser-384 is essential for chromosome segregation fidelity and genomic stability.

Mechanistic Insight into AP-Endonuclease 1 Cleavage of Abasic Sites at Stalled Replication Forks

This study investigates APE1 cleavage of abasic sites at replication forks, revealing its mechanism and interaction with RPA.

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

Computational and biochemical analyses reveal that cofilin-2 self assembles into amyloid-like structures and promotes the aggregation of other proteinaceous species: Pathogenic relevance to myopathies

This study shows cofilin-2 can form amyloid-like structures and promote protein aggregation, suggesting a role in myopathies.

Discovery of a first-in-class inhibitor of the PRMT5-substrate adaptor interaction

The paper identifies a first-in-class inhibitor, BRD0639, that targets the PRMT5-PBM interaction, disrupting SAP binding and reducing substrate methylation in cancer cells.