Author

S. Kuppa

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 6 Biophysics· 1 Cancer Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:human· 6 Assay:proteomics· 5 Research Focus:chromatin & epigenetic regulation· 4 Research Focus:cell signaling & trafficking· 3 Research Focus:protein folding & dynamics· 3 Approach:biophysical modeling· 1 Research Focus:development & morphogenesis· 1

Frequent Co-Authors

E. Antony· 8 shared V. Kaushik· 5 shared B. Bothner· 3 shared J. Mattice· 3 shared N. Pokhrel· 3 shared R. Chadda· 3 shared A. Patterson· 2 shared P. Roshan· 2 shared

Activity by Year

2021-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 43 mutual co-author links

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8 shared papersBiochemistry

E. Antony

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions

S. Pangeni, G. Biswas, V. Kaushik, S. Kuppa, O. Yang, C.-T. Lin, G. Mishra, Y. Levy, E. Antony, T. Ha

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.

bioRxiv · Dec 9, 2023Biophysics

Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

S. Kuppa, E. Corless, C. C. Caldwell, M. Spies, E. Antony

This study generates a fluorescently labeled human XPA protein to study DNA binding dynamics during nucleotide excision repair.

bioRxiv · Nov 23, 2023Biochemistry

Fluorescent human RPA to track assembly dynamics on DNA

V. Kaushik, R. Chadda, S. Kuppa, N. Pokhrel, A. Vayyeti, S. Grady, C. Arnatt, E. Antony

This paper describes fluorescently modified human RPA to study its dynamic interactions with ssDNA and other proteins.

bioRxiv · Nov 23, 2023Biochemistry

Interplay of macromolecular interactions during assembly of human DNA polymerase δ holoenzymes and initiation of DNA synthesis

J. L. Norris, L. O. Rogers, K. G. Pytko, R. L. Dannenberg, S. Perreault, V. Kaushik, S. Kuppa, E. Antony, M. Hedglin

This study uses FRET assays to show how RPA interactions at P/T junctions facilitate Pol δ holoenzyme assembly and DNA synthesis efficiency.

bioRxiv · May 9, 2023 · PreprintBiochemistry

An Aurora B-RPA signaling axis secures chromosome segregation fidelity

P. Roshan, S. Kuppa, J. Mattice, V. Kaushik, R. Chadda, N. Pokhrel, B. Tumala, B. Bothner, E. Antony, S. Origanti

This study identifies a mitosis-specific regulation of RPA by Aurora B kinase, showing that phosphorylation of RPA70 at Ser-384 is essential for chromosome segregation fidelity and genomic stability.

bioRxiv · Sep 26, 2022 · PreprintBiochemistry

Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunit.

J. Elliff, A. Biswas, S. Kuppa, P. Roshan, R. Burd, M. Chinnaraj, A. Patterson, J. Mattice, S. Walker, N. Pozzi, E. Antony, B. Bothner, S. S. Origanti

This study identifies key residues in eIF6 and RPL23 that mediate their interaction and shows how mutations in SDS disrupt this interaction, offering a therapeutic target for cancer and SDS.

bioRxiv · Jul 29, 2022Biochemistry

Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins

S. Kuppa, J. Deveryshetty, R. Chadda, J. Mattice, N. Pokhrel, V. Kaushik, A. Patterson, N. Dhingra, S. Pangeni, M. K. Sadauskas, S. Shiekh, H. Balci, T. Ha, X. Zhao, B. Bothner, E. Antony

This study shows Rtt105 binds to RPA, staples it, and blocks RIP interactions without ssDNA, while stabilizing RPA-ssDNA filaments with ssDNA.

bioRxiv · Feb 5, 2022 · PreprintBiochemistry

A-type lamins are critical for the recruitment of RPA and RAD51 to stalled replication forks to maintain fork stability

S. Graziano, N. Coll-Bonfill, B. Teodoro-Castro, S. Kuppa, J. Jackson, E. Shashkova, U. Mahajan, A. Vindigni, E. Antony, S. Gonzalo

This study shows A-type lamins are essential for recruiting RPA and RAD51 to stalled replication forks, preventing replication fork instability during replication stress.

bioRxiv · Jun 22, 2021 · PreprintCancer Biology