M. Kloos

Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography

The paper determines the crystal structure of a bacterial photoactivated adenylate cyclase at room temperature using serial femtosecond crystallography and compares it with cryo structures to study structural changes upon light exposure.

Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography

This paper introduces a mix-and-extrude method for time-resolved protein crystallography using 3D printed microchannels to study ligand-induced reactions in viscous media.

Influence of pump laser fluence on ultrafast structural changes in myoglobin

This study shows that varying pump laser fluence in TR-SFX experiments affects structural dynamics and coherent oscillations in myoglobin, highlighting the need for linear photoexcitation in ultrafast studies.

Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement

This paper enhances switching contrast in photoswitchable fluorescent proteins by modifying the V151 side chain, improving performance in RESOLFT nanoscopy.

Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX

This paper determines the structure of Tpp49Aa1 using MHz-SFX and investigates its interaction with Cry48Aa1, expanding its known target range to include additional mosquito species.

Direct Observation of the Mechanism of Antibiotic Resistance by Mix-and-Inject at the European XFEL

The paper directly observes fast ligand binding in a biomedically relevant enzyme using MHz X-ray pulses at the European XFEL.