M. Pelosse

biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locus

The paper introduces biGMamAct, a system for efficiently integrating large DNA cargoes at the ACTB locus using CRISPR/Cas9, demonstrating its use in expressing multiple fluorescent proteins and visualizing subcellular compartments.

FRET-based sensor for measuring adenine nucleotide binding to AMPK

The paper describes an AMPK-based FRET sensor, AMPfret, that detects conformational changes in AMPK upon nucleotide binding, enabling analysis of AMPK activation in vitro and in cells.

Structural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family

This study characterizes the structure and function of the minimal orthomyxovirus-like polymerase from Tilapia Lake Virus, revealing its active domains and inactive features.

Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation

The paper determines the structure and activation mechanism of the MKK6-p38α complex using cryo-EM and MD simulations, revealing how phosphorylation and interactions drive MAPK specificity.