M. Voorhies

Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma

This paper develops a CRISPR/Cas9 system for efficient targeted gene disruption in Histoplasma, enabling high-efficiency mutagenesis and deletion of specific genes.

Chemical stimuli override a temperature-dependent morphological program by reprogramming the transcriptome of a fungal pathogen.

This study identifies transcription factors that reprogram the transcriptome of Histoplasma to induce hyphal growth at 37°C, revealing a regulatory circuit involving STU1, FBC1, and PAC2.

A Legionella toxin mimics tRNA and glycosylates the translation machinery to trigger a ribotoxic stress response.

This paper identifies SidI, a Legionella toxin that mimics tRNA, glycosylates the translation machinery, and triggers a ribotoxic stress response leading to cell death.

Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages

This study uses CRISPR screening to identify C3aR as critical for macrophage capture of fungi, revealing a novel role in fungal pathogen recognition.

The ESX-1 secretion system senses bacterial contact and prepares mycobacteria for environmental adaptation

The paper shows the ESX-1 system in mycobacteria senses bacterial contact and environmental nitrogen levels, regulating secretion and adaptation.

Cbp1, a rapidly evolving fungal virulence factor, forms an effector complex that drives macrophage lysis

This paper identifies Cbp1 as a rapidly evolving fungal virulence factor that localizes to the host cell cytosol and forms a complex to drive macrophage lysis.

The WOPR family protein Ryp1 is a key regulator of gene expression, development, and virulence in the thermally dimorphic fungal pathogen Coccidioides posadasii

This study identifies Ryp1 as a key transcription factor regulating spherule formation and virulence in Coccidioides posadasii through transcriptional profiling and mutant analysis.

Chromosome-level genome assembly of a human fungal pathogen reveals synteny among geographically distinct species

This study presents chromosome-level genome assemblies of five Histoplasma strains, revealing synteny and gene expression patterns across geographically distinct isolates.

Protomer alignment modulates specificity of RNA substrate recognition by Ire1

The paper shows that changing two amino acids in S. cerevisiae Ire1 to their S. pombe counterparts makes it promiscuous, revealing that protomer alignment modulates RNA substrate specificity.

Opposing signaling pathways regulate morphology in response to temperature in the fungal pathogen Histoplasma capsulatum

This study identifies temperature-responsive regulons in Histoplasma capsulatum that regulate morphology and gene expression in response to temperature, revealing opposing signaling pathways that control hyphal and yeast forms.