CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos
Gopal Kushawah, Luis Hernández-Huertas, Joaquín Abugattas-Núñez del Prado, Juan Ramón Martínez‐Morales, Michelle Lynn DeVore, Huzaifa Hassan, Ismael Moreno-Sánchez, Laura Tomás‐Gallardo, Alejandro Díaz‐Moscoso, Dahiana E. Monges, Javier R. Guelfo, William C. Theune, Emry O. Brannan, Wei Wang, Timothy J. Corbin, , Miguel A. Moreno-Mateos
This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.
Developmental Cell · Aug 7, 2020Peer-reviewedDevelopmental Biology