William C. Theune

Transcriptomic profiling of retinal cells reveals a subpopulation of microglia/macrophages expressing Rbpms and Spp1 markers of retinal ganglion cells (RGCs) that confound identification of RGCs

The paper identifies Rbpms+ and Spp1+ microglia/macrophages in scRNA-seq data that mimic RGC markers, confounding RGC identification and proposes solutions to address this issue.

CellTools algorithm for mapping scRNA-seq query cells to the reference dataset improves the classification of resilient and susceptible retinal ganglion cell types

The paper introduces CellTools, an algorithm that improves scRNA-seq cell mapping and classification, revealing new insights into retinal ganglion cell resilience to injury.

CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos

This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.