R. B. Best

Exploring the Sequence Fitness Landscape of a Bridge Between Protein Folds

The paper develops a computational model to predict fold-switching sequences between GA and GB domains of protein G, identifying pathways and showing that low stability sequences are more likely to switch folds.

The ribosome modulates folding inside the ribosomal exit tunnel

The paper investigates how the ribosomal exit tunnel affects the folding of a small zinc-finger domain, finding that electrostatic interactions, not just confinement, contribute to folding acceleration and stabilization.

Disordered Proteins Enable Histone Chaperoning on the Nucleosome

The paper shows that disordered regions in proteins like H1 enable competitive displacement by histone chaperones such as prothymosin on the nucleosome.

Cotranslational folding cooperativity of contiguous domains of α-spectrin

The paper investigates how adjacent domains of α-spectrin influence each other's cotranslational folding and estimates the mechanical force exerted during this process.

IMPROVING THE PERFORMANCE OF THE AMBER RNA FORCE FIELD BY TUNING THE HYDROGEN-BONDING INTERACTIONS

This paper improves the AMBER RNA force field by tuning hydrogen-bonding interactions to better match experimental data in RNA folding simulations.

The Shape of the Ribosome Exit Tunnel Affects Cotranslational Protein Folding

The study shows that the geometry of the E. coli ribosome exit tunnel influences where proteins fold during translation, with larger domains folding deeper when tunnel space is available.

A small single-domain protein folds through the same pathway on- and off- the ribosome

This study shows the src SH3 domain folds through the same pathway whether on or off the ribosome, suggesting the ribosome does not alter its folding mechanism.

Reversible two-state folding of the ultrafast protein gpW under mechanical force

This study uses single-molecule AFM to show that the ultrafast protein gpW folds and unfolds stochastically under mechanical force, revealing a force-induced folding barrier.

Sequence determinants of protein phase behavior from a coarse-grained model

This paper develops a coarse-grained computational model to simulate liquid-liquid phase separation of proteins, showing it can capture phase diagram changes due to mutations and domain presence.