Author

R. Lei

Genapsys (United States)

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 4 Biochemistry· 2 Evolutionary Biology· 1 Genomics· 1 Immunology· 1 Plant Biology· 1

Signature Assays, Organisms & Methods

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Assay:proteomics· 6 Approach:deep learning· 5 Approach:experimental assay· 5 Organism:human· 4 Research Focus:drug response / drug discovery· 3 Research Focus:chromatin & epigenetic regulation· 2 Research Focus:host-pathogen & immune response· 2 Organism:virus· 2

Frequent Co-Authors

N. C. Wu· 8 shared T. J. C. Tan· 6 shared K. A. Matreyek· 3 shared W. O. Ouyang· 3 shared Y. Wang· 3 shared A. Odle· 2 shared C. Kieffer· 2 shared C. S. Graham· 2 shared

Activity by Year

2020-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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8 shared papersMicrobiology

N. C. Wu

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

Functional and antigenic characterization of SARS-CoV-2 spike fusion peptide by deep mutational scanning

R. Lei, E. Qing, A. Odle, M. Yuan, T. J. C. Tan, N. So, W. O. Ouyang, I. A. Wilson, T. Gallagher, S. Perlman, N. C. Wu, L. Y. R. Wong

This study uses deep mutational scanning to analyze the SARS-CoV-2 spike fusion peptide, identifying mutations affecting viral entry and antibody resistance.

bioRxiv · Nov 28, 2023Microbiology

Evidence of antigenic drift in the fusion machinery core of SARS-CoV-2 spike

T. J. C. Tan, A. Odle, R. Lei, K. A. Matreyek, S. Perlman, L.-Y. R. Wong, N. C. Wu

This study identifies S2 domain mutations in SARS-CoV-2 spike that reduce binding to antibodies, showing antigenic drift in this region and its impact on vaccine efficacy.

bioRxiv · Sep 27, 2023Microbiology

An explainable language model for antibody specificity prediction using curated influenza hemagglutinin antibodies

Y. Wang, H. Lv, R. Lei, Y.-H. Yeung, I. R. Shen, D. Choi, Q. W. Teo, T. J. C. Tan, A. B. Gopal, X. Chen, C. S. Graham, N. C. Wu

This study curates a dataset of influenza HA antibodies and develops an explainable language model to predict antibody specificity, identifying and validating HA stem antibodies.

bioRxiv · Sep 11, 2023 · PreprintImmunology

Imprinted anti-hemagglutinin and anti-neuraminidase antibody responses after childhood infections of A(H1N1) and A(H1N1)pdm09 influenza viruses

P. Daulagala, B. Mann, K. Leung, E. Lau, L. Yung, R. Lei, S. I. N. Nizami, J. T. Wu, S. S. Chiu, R. S. Daniels, N. C. Wu, D. E. Wentworth, J. S. M. Peiris, H.-L. Yen

The paper characterizes anti-HA and anti-NA antibody responses to A(H1N1) and A(H1N1)pdm09 influenza viruses in individuals born between 1950-2015, finding age-dependent imprinting and cross-reactivity to multiple antigenic variants.

bioRxiv · Feb 1, 2023Microbiology

High-throughput identification of prefusion-stabilizing mutations in SARS-CoV-2 spike

T. J. C. Tan, Z. Mou, R. Lei, W. O. Ouyang, M. Yuan, G. Song, R. Andrabi, I. A. Wilson, C. Kieffer, X. Dai, K. A. Matreyek, N. C. Wu

The paper identifies mutations that stabilize the prefusion conformation of SARS-CoV-2 spike while improving expression, using a combination of fluorescence-based assays, cell display, and deep mutational scanning.

bioRxiv · Sep 24, 2022Biochemistry

Probing the biophysical constraints of SARS-CoV-2 spike N-terminal domain using deep mutational scanning

W. O. Ouyang, T. J. C. Tan, R. Lei, G. Song, C. Kieffer, R. Andrabi, K. A. Matreyek, N. C. Wu

This study quantifies the effects of NTD mutations on S protein expression and identifies mutations that enhance expression without altering antigenicity.

bioRxiv · Jun 20, 2022Biochemistry

Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase

R. Lei, T. J. C. Tan, A. H. Garcia, Y. Wang, C. Teo, G. Gopan, Z. Tavakoli Dargani, Q. W. Teo, C. S. Graham, S. K. Nair, N. C. Wu

The paper identifies deleterious mutations in human influenza H3N2 neuraminidase and reveals epistatic networks shaping its evolution.

bioRxiv · Feb 24, 2022Microbiology

Antigenic evolution of human influenza H3N2 neuraminidase is constrained by charge balancing

Y. Wang, R. Lei, A. Nourmohammad, N. C. Wu

This study uses deep mutational scanning to show that charge balancing constrains antigenic evolution in H3N2 neuraminidase.

bioRxiv · Jul 10, 2021Evolutionary Biology

High accuracy DNA sequencing on a small, scalable platform via electrical detection of single base incorporations

Hesaam Esfandyarpour, Kosar B. Parizi, Meysam R. Barmi, H.R. Rategh, Lisen Wang, Saurabh Paliwal, HamidReza Golnabi, Paul Kenney, Richard Reel, Frank Lee, Xavier V. Gomes, Seth Stern, Ashok Ramachandran, Subra Sankar, Solomon Doomson, , Ronald Wayne Davis

This paper introduces an electronic detection-based DNA sequencing platform that achieves high accuracy and scalability for microbial and human genome sequencing.

bioRxiv · May 30, 2020 · PreprintGenomics

bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

Y. Li, R. Lei, M. Pu, Y. Cai, C. Lu, Z. Li, G. Liang

The paper identifies bHLH11 as a negative regulator of Fe homeostasis in Arabidopsis, showing it inhibits bHLH IVc transcription factors by recruiting TOPLESS/TOPLESS-RELATED corepressors.

bioRxiv · Apr 9, 2020 · PreprintPlant Biology