Rebecca S Shapiro

This study shows that non-homologous end joining (NHEJ) facilitates rapid adaptation to caspofungin in Nakaseomyces glabratus by enabling FKS2 mutations, while NHEJ-deficient strains adapt less efficiently through alternative pathways.

This paper introduces a method to tune guide RNA for precise Cas9 targeting, preventing mutations in engineered organisms and demonstrating scalability and robustness across Cas9 variants and environments.