Nicholas C Gervais

Non-homologous end-joining is implicated in rapid and efficient caspofungin adaptation in Nakaseomyces glabratus

This study shows that non-homologous end joining (NHEJ) facilitates rapid adaptation to caspofungin in Nakaseomyces glabratus by enabling FKS2 mutations, while NHEJ-deficient strains adapt less efficiently through alternative pathways.

CRISPR-activation reveals key resistance genes and vulnerabilities of copy number variants in Candida albicans

This study uses CRISPR-activation to identify genes with fitness effects in Candida albicans isolates, revealing resistance genes and vulnerabilities associated with copy number variants.

Chromosome-scale CRISPR screening reveals secretory pathway genes as drivers of aneuploidy-mediated antifungal tolerance

The paper uses CRISPR screening to identify secretory pathway genes that drive antifungal tolerance through aneuploidy in Candida albicans.

The Unique Efg1 Fungal Virulence Regulon in the Catheterized Bladder Environment

This study identifies the Efg1 regulon active in C. albicans during growth in human urine, validates its clinical relevance in catheter samples, and characterizes key downstream factors involved in fungal CAUTI.

Pooled CRISPRi screening reveals fungal-specific vulnerabilities across environments and genetic backgrounds

This study uses pooled CRISPRi screening in Candida albicans to identify dosage-sensitive genes across diverse environments and genetic backgrounds, highlighting potential antifungal targets.

HyperdCas12a-Based Multiplexed Genetic Regulation in Candida albicans

This paper introduces a hyperdCas12a-based CRISPR system for multiplexed gene regulation in Candida albicans, demonstrating its effectiveness in studying genetic interactions and drug resistance.

FungAMR: A comprehensive portrait of antimicrobial resistance mutations in fungi

The paper curates AMR mutations in fungi to create FungAMR, identifies universal resistance mutations, and provides a tool to screen genomes for AMR mutations.

Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9

This paper uses CRISPR-Cas9 to induce targeted loss of heterozygosity in Candida albicans and validates its role in antifungal resistance.